Fig. 7. Adult phenotypes of ultraspiracle and baboon PPN clones. (A1) Wild-type PPNs labeled by GH146 MARCM typically innervate a single glomerulus in the adult AL and (A2) have several collateral branches with terminal boutons in the MB calyx and a characteristic branching pattern in the LH (see also Fig. 1). (B1,C1) usp³ PPN dendrites generally target an appropriate glomerulus in the AL but also exhibit a few ectopic processes (arrows), while (B2,C2) their axons often retain large larval-like synaptic structures directly on the main trunk (arrowheads). The axon trunk sometimes makes a detour from the main inner antennocerebral tract within MB to connect these larval-like boutons (C2 only). Axon termini in the LH are morphologically normal, although they may include possible extra branches, which might be putative larval remnants (B2, blunt arrow). (D1-F1) babo^Fd4 PPN dendrites often exhibit more severe phenotypes than that of usp³; they can be sparse and localized to a few areas in the AL without targeting a specific glomerulus (D1), leave the AL entirely (E1, arrow), or be broadly distributed throughout the AL (F1). (D2-F2) All babo^Fd4 PPN axons exhibit larval-like boutons directly on their main trunks in the MB calyx (arrowheads) and occasionally detour from the inner antennocerebral tract to connect them (F2 only). Additional defects seen in the LH include unusually profuse swellings along the branches (D2), failure to enter the LH (E2), and failure to elaborate axon termini (F2). Note: All AL and MB/LH images are presented in pairs from the same animal, but this is not meant to imply consistent correlations between the various dendritic and axonal phenotypes. (G) Quantification of usp³ and babo^Fd4 phenotypes in the adult AL. Categories are `WT' (uniglomerular dense dendrites), `glom + extra' (uniglomerular dendrites plus extra processes in the AL), `sparse local' (sparse dendrites localized to a region of the AL but not innervating any particular glomerulus), `sparse broad' (sparse dendrites distributed broadly throughout the AL) and `leaves AL' (dendrites arborize outside the AL proper). Note that although the first four categories are mutually exclusive, the fifth can overlap with any of the others except for `WT'. (H) Quantification of usp³ and babo^Fd4 phenotypes in the adult MB calyx. Categories are `WT' (adult-like collateral branches with terminal swellings), `leaves iACT' (main axon trunk detours from the inner antennocerebral tract, usually to connect with a larval-like bouton), `larval + adult boutons' (both larval-like boutons on the main axon trunk and adult-like collateral branches), and `larval boutons only' (larval-like boutons on the main axon trunk but no adult-like collateral branches). Note that `leaves iACT' can overlap with either of the last two categories; the others are mutually exclusive. (I) Quantification of usp<sup>3</sup> and babo<sup>Fd4</sup> phenotypes in the adult LH. Categories are `WT' (terminal branches of varying complexity in LH), `swellings' (unusually profuse swellings along the terminal branches), `LH entry' (failure to enter the LH proper), and `elaboration' (failure to elaborate second order terminal branches in the LH). Note that there is overlap between all categories except for `WT'.