Fig. 3. eff-1 expression is repressed by ceh-16 in the embryonic seam cells. (A) ceh-16(lg16) mosaic for the rescue marker ceh-16::gfp shows seam cell fusions (arrows) and cell migrations (asterisk) (compare Fig. 2). (B) Mosaic animal as in A crossed into eff-1(hy21); suppression of fusion (arrows). (C) eff-1(hy21) animal transgenic for an eff-1::gfp transcriptional construct that is expressed in cells committed to fuse. (D) as in C, but in addition this animal is ceh-16(RNAi). Ectopic eff-1::gfp expression (de-repression) and ectopic migration in the seam cells (arrows) as a result of ceh-16 inactivation. (E-G) ceh-16(RNAi) embryo transgenic for eff-1::gfp taken at three time points: eff-1 expression (eff-1p::gfp) in seam cells is de-repressed, resulting in fusion (arrows). All embryos are transgenic for ajm-1::gfp. All animals are at 1.5-fold stage except for E (bean stage). Scale bars: 10 µm.