Fig. 2. Testing GATA4, 5 and 6 for direct induction of endoderm markers in animal caps. Animal caps expressing HA-tagged Xenopus GATA4, 5 or 6 fused to the glucocorticoid receptor ligand-binding domain cultured in the presence or absence of dexamethasone (Dex) and/or cycloheximide (CHX) (A-C) or Emetine (EM) (D). Radiolabelled methionine incorporation measured by TCA precipitation (A) and gel electrophoresis (B). Real-time RT-PCR of Sox17, HNF1ß or HNF3ß (C,D). For error bars and Erk2 see legend to Fig. 1. (E) The data are represented as a simplified network of interactions, where Xs represent hypothetical intermediate factors, dotted lines are possibly indirect inductions and solid lines are direct inductions.