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Fig. 3. GATA4 induces early endoderm markers via GATA6. Stage 12 animal caps injected with HA-tagged Xenopus GATA4, 5 or 6 (50 pg) with or without GATA4, 5 or 6 MOs (5 ng). Protein production (B) and real-time RT-PCR for GATA4, 5 and 6 (A) and Sox17{alpha}, HNF1ß (C,D). LG6: long GATA6, SG6: short GATA6, G5: GATA5, G4: GATA4. For Erk2 and error bars, see Fig. 1 legend. GATA factors represent some of the hypothetical intermediates (`X') in Fig. 2E (E). Xs, dotted and solid lines, as in Fig. 2 legend.