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Fig. 4. Activin induces Sox17{alpha} via GATA4, 5 and 6 in animal caps. Stage 12 animal caps injected with activin RNA and GATA4, 5 or 6 MOs. Real-time RT-PCR for Sox17{alpha} (A,B) or HNF1ß and endogenous GATA4, 5 and 6 (C). Data summarised in (D). Shaded box represents the temporal and/or spatial activin/nodal gradient of activity thought to develop in early embryos. Xs, dotted and solid lines as in Fig. 2 legend.