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Fig. 5. GATA4, 5 and 6 are required for development of the endoderm in vivo. (A,B,D) In situ hybridisation of Sox17{alpha} (A,B) and HNF1ß (D) in stage 12 embryos injected vegetally with GATA4, 5 or 6 MOs (10-40 ng). (A,D) First column shows typical whole mount images and second column shows internal staining in halved embryos, black line indicating the plane of section. Yolk plug (YP) and involuted endoderm (blue arrows) are indicated. Black arrows in (D) indicate mesodermal expression. The percentages of embryos expressing high (normal), medium or low levels of Sox17{alpha} are represented in a histogram (B). (C,E) Real time RT-PCR analysis of Sox17{alpha} (C) and HNF1ß and HNF3ß (E). For error bars see Fig. 1 legend. (F) In situ hybridisation of HNF1ß in stage 34 embryos. Position of the liver anlagen is indicated. (G) General gut morphology in stage 43 embryos. Embryos injected with 40 ng GATA6 MO did not survive to stage 43 and therefore the 20 ng phenotype is shown. The single embryo surviving up to stage 43 of the 30 ng GATA4, 5 and 6 MO injection is shown.