Fig. 2. VISTA data of MFCS1. The VISTA search for mouse-human and mouse-medaka homologous sequence revealed the full extent and core sequence of the MFCS1. In the VISTA search, we used 50 bp window length and 75% conservation level. The sites of two mouse mutations (red arrows), Hx and M100081, and four human PPD mutations (black arrows) are superimposed on MFCS1. A DNA segment (1167 bp in length) that includes the entire MFCS1 was eliminated by gene targeting in this study.