Fig. 6. Expression patterns of marker genes in the limb buds at E10.5. In all figures except for A, D and G, anterior side is top. In wild-type (A-C) and MFCS1 KO heterozygous (D-F) embryos, Shh expression is observed in the posterior mesenchyme of the limb buds. In the limb buds of MFCS1 KO homozygous embryos (G-I), Shh expression is not detected. Expression of marker genes, dHand (J-M) and Gli3 (N-Q), Fgf8 (R-U) and Fgf4 (V-Y) were examined at a stage prior to Shh expression.