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Fig. 10. Addition of LPA to dissociated cells increases the density of the cortical actin network. (A) Cells treated for 5 minutes with 1 µm LPA show an increase in the density of cortical actin compared with control cells. Scale bar: 5 µm. (B) Quantitation of the levels of cortical actin in individual dissociated cells measured by pixel intensity of Alexa 488-phalloidin fluorescence. Bars 1 and 6 represent dissociated cells, not treated with LPA, at the beginning and end of the experiment respectively. Bars 2-5 represent cells treated for 5 minutes with LPA, which was then washed out, and cells fixed 0, 15, 30 and 45 minutes later. Asterisks represent a significant difference (P<0.05) compared with control.