Fig. 4. Quantitation of changes in the intensity of phalloidin staining. There is a significant increase in the intensity of phalloidin staining if caps are treated with LPA (A) or if either LPA receptor is overexpressed in caps (B), but not with treatment of the related phospholipid phosphatidic acid (A). (C,D) There is a significant decrease in the intensity of phalloidin staining by targeting the mRNA with either a phosphorothioate oligo or morpholino oligonucleotides. Asterisks indicate significance at P<0.05. All data are representative of a single experiment and all experiments were repeated at least three times with five caps per group.