Fig. 6. Overexpression of either X. tropicalis XLPA₁ or XLPA₂ mRNA causes an increase in cortical actin and more rapid wound healing, mimicking LPA treatment. (A) The bases (after excision of animal caps) of embryos injected with 400 pg of XLPA₁ elongated along the animal-vegetal axis after the vitelline membrane was removed and were more rigid than control embryos. These bases healed faster than controls. Scale bar: 350 µm. (B) Bases from embryos injected with XLPA₂ mRNA showed a similar phenotype as bases from XLPA₁-injected embryos. Scale bar: 450 µm. (C) Animal caps from XLPA₂-injected embryos rounded up faster than control caps after 10 minutes of healing. Scale bar: 125 µm. Injection of either XLPA₁ (D) or XLPA₂ (E) mRNA increased the levels of cortical actin in the animal cap cells and in the purse-string compared with controls. Scale bar: 50 µm.