Fig. 4. sqRT-PCR examination of gene expression in Lfng^–/– ovaries. sqRT-PCR with gene-specific primers was performed to detect transcripts in total ovary RNA from 42-day-old mice of all three genotypes (Lfng^+/+, Lfng^+/– and Lfng^–/–). Three samples per genotype were normalized against the ribosomal gene L7 and representative data are presented. N2, Notch2; N3, Notch3; J1, Jagged1; J2, Jagged2; EP2, prostaglandin type 2 receptor (Ptger2 – Mouse Genome Informatics); Fshr, follicle stimulating hormone receptor.