Fig. 5. Expression of Notch family genes in the Lfng^–/– ovary by ISH. Gene-specific digoxigenin labeled probes were used, as indicated; representative data from a minimum of three replicates of Lfng^+/– and Lfng^–/– are presented. Notch3 demonstrated no change in follicle stage or cell-type-specific expression in null ovaries; black arrows indicate granulosa cell staining and the red arrowhead indicates expression in an early CL. Jagged1 was limited to oocytes of small growing follicles in the Lfng^–/– ovary (arrowhead). Expression of Notch downstream target genes Hes5, Hes1, Hesr1 and Hesr2 was not detected in the Lfng^–/– follicles (only representative family members Hes5 and Hesr2 are presented). Note positive follicles indicated by arrows in +/– and compare with similarly sized follicles indicated by arrowheads in –/–.