Fig. 6. Expression of other follicle-specific genes. Connexin43 (Cx43) was detected by IHC using an alkaline phosphatase-conjugated secondary antibody and DAB substrate, as indicated by the red stain in granulosa cells and oocytes. There was no alteration in Cx43 expression. Kit ligand is expressed normally in Lfng^–/– granulosa cells. Kit ligand transcripts were detected by ISH, note the purple stain in granulosa cells, dark field microscopy. Kit receptor (c-Kit) was also unaffected in Lfng null follicles. Kit receptor was detected by IHC, arrowhead in +/– indicates a group of primary follicles with positive oocytes. Controls included no primary antibody, note the lack of red stain.