Fig. 3. RhoGEF2 and nullo/sry- cooperate in furrow canal formation. (A-E) Embryos from RhoGEF2^1.1 germline clones were injected at the posterior pole with the following dsRNA: (A) Bsg25D, (B,E) nullo, (C,D) sry-, and stained for DNA (blue), F-actin (green), Sry- (red). Loss of Sry- protein and loss of furrow canals (marked by F-actin, optical transversal sections, D,E) correlates with the posterior site of dsRNA injection as shown by respective antibody staining (C). Scale bars: (A-C) 50 µm, (D,E) 10 µm. Posterior to the right, arrow indicates injection site.