Fig. 1. Expression of XGrhl1 is restricted to the ectodermal layer. (A) RT-PCR analysis of XGrhl1 expression in staged embryos during development (n=5). Ornithine decarboxylase (ODC) gene expression was used as a control for RNA concentration. -RT, no reverse transcriptase; -, no RNA. (B) XGrhl1 expression is restricted to the superficial epidermal layer of the developing embryo. RT-PCR analysis of XGrhl1 and developmental gene expression in different ectodermal tissues at stage 11 of Xenopus development.