Fig. 1. UBC-9 binds two consensus sumoylation motifs of LIN-1. (A) Schematic of LIN-1: ETS DNA-binding domain (black) and consensus sumoylation motifs (above); the D domain (D) and the FQFP motif (F) are docking sites for ERK. The positions of the e1275 and n1790 mutations and amino acid numbers are shown below. (B) The interaction of GAL4AD:UBC-9(1-166) with the indicated LexA DNA-binding domain (LA):LIN-1 fusion protein was monitored using the two-hybrid system. Bars represent the average LexA-dependent ß-galactosidase activity from at least five independent yeast transformants and lines indicate the standard deviation. The values were normalized by setting the interaction with LA:LIN-1(1-252) equal to 100 RLU.