Fig. 2. Intra-embryonic hematopoiesis is impaired in the RBPj mutant embryos and they display an aberrant expression of Notch family members in the aorta (A) Semiquantitative RT-PCR analysis in dissected P-Sp of E9.5 wild-type and RBPj mutants. Representative PCR products after 35 and 40 cycles of two independent experiments are shown. (B) WISH, with the indicated probes and transverse sections of E9.5 aortas of RBPj mutants. Orientation of the aortas is dorsal (up) to ventral (down). (C) Hematopoietic CFC from dissected P-Sp of E9.5 wild-type and RBPj mutants. Bars represent the average number of CFCs and standard deviation from three different embryos. (D) Liquid cultures with IL3 and SCF-conditioned media from P-Sp of E9.5 wild-type and RBPj mutants at days 0 and 6. (E) After 6 days in culture, cells were assayed for the expression of CD45 by flow cytometry and (F) the number of CFCs generated. Bars represent the average number of CFCs obtained from one wild-type embryo and pools of two or three mutant embryos equivalent (e.e.) in three independent different experiments.