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Fig. 3. Absence of hematopoietic cells and increase of endothelial cells in the P-Sp/AGM of RBPj{kappa} mutant embryos. (A) Semiquantitative RT-PCR analysis in dissected P-Sp of E9.5 wild-type and RBPj{kappa} mutants. Representative PCR products after 35 and 40 cycles of two independent experiments are shown (B) WISH of hematopoietic transcription factors and transverse sections of E9.5 aortas of wild-type and RBPj{kappa} mutants. (C) WISH with VE-C (upper panel), expression of VE-C in the aorta (middle panels) and expression of PECAM/CD31 by immunofluorescence (lower panel) on transverse sections of E9.5 aortas of wild-type and RBPj{kappa} mutants. Orientation of the aortas is dorsal (up) to ventral (down).