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Fig. 5. Notch1+/Gata2+ cells in the P-Sp/AGM endothelium are Jag1+/Jag2-. Double in-situ hybridization on transverse section of wild-type E10.5 aortas. (A) Hybridization of Notch1 with Jag1 (upper), Jag2 (middle) and Dll4 (lower panels). Representative photographs of at least three hybridizations are shown (B) Model for Notch function in the formation of hematopoietic clusters from the aorta endothelium during development.