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Fig. 3. X-gal staining in transient transgenic embryos shows endocardial-specific enhancer activity of the 4.1 kb BssHII-BssHII P2 fragment in whole-mount E9.5 embryos (A) and sections of whole-mount-stained E10.5 (B) and E11.5 (C) embryos. ß-Galactosidase activity is restricted to the pro-valve endocardial cells in AVC (arrowhead) and OFT (arrow). The enhancer is not activated in the mesenchymal cells derived from transformed endocardial cells in the AVC and OFT endocardial cushions. Top row, OFT; bottom row, AVC. a, atrium; c, conus; t, truncus; v, ventricle.