Fig. 5. Edar expression correlates with ß-catenin pathway activation. (A,B) Detection of Edar transcripts (A) and ß-catenin protein (B) on adjacent sections of skin from an embryo at day 8 of incubation. Nuclei are stained red and nuclear ß-catenin appears yellow. In lateral regions (left), Edar is not detected and no accumulation of ß-catenin in the nuclei is observed (left inset). In more medial regions, a band of Edar expression correlates with moderate nuclear accumulation of ß-catenin (middle inset). At the dorsal midline, nascent buds (right inset, yellow bar) express high levels of Edar and nuclear accumulation of ß-catenin, whereas adjacent interplacodal epidermis does not (right inset, red bar). (C-E) Adjacent sections from skin harvested at day 10 show that Eda expression in the bud dermis (E) corresponds to regions of nuclear ß-catenin accumulation (D), whereas Edaradd expression in the epidermis of the bud does not (C).