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Fig. 7. Fgf15 is a potential downstream target of Bmp signaling in the retina. (A) Fgf15 is strongly expressed in the distal optic vesicle at E9.0-9.5 in a wild-type embryo. (B) The expression of Fgf15 is absent in the eye (arrowhead) of an advanced Bmp4-/- mutant embryo with equivalent number of somites to the wild-type embryo shown in A. (C,D) Application of BMP4-soaked beads (asterisks) restores the expression of Fgf15 in the distal optic vesicle of Bmp4-/- by 18 hours in culture (C, arrowhead). Under the same condition, BSA fails to restore the expression of Fgf15 (D, arrowhead). Arrows indicate the future retinal pigmented epithelium slightly displaced anteriorly during explant culture. (E) Fgf15 expression persists through later stages as shown for an E11.5 normal retina (Bmpr1a+/fx;Bmpr1b+/-;Cre control). (F) In Bmpr1a-/fx;Bmpr1b-/-;Cre double mutant, the expression of Fgf15 is absent (arrows). le, lens; ov, optic vesicle. Scale bar: 50 µm in A-D; 100 µm in E,F.