Fig. 4. Establishment of primary cultures of spleen erythroblasts in proliferation conditions. (A) Freshly dissociated mouse splenocytes obtained from 10-day-old wild-type mice were stained with anti-CD71 and anti-TER119 antibodies to estimate the amount of TER119⁺/CD71⁺ erythroblasts before plating in serum-free medium under conditions that favor proliferation of erythroblasts. (B) Cumulative cell number of erythroblasts determined daily by Trypan Blue exclusion. (C) MGG and neutral benzidine staining of cytospin preparations obtained from proliferating erythroblasts analyzed on day 7.