Fig. 6. Erythropoietic reconstitution in lethally irradiated mice. Donor spleen
cells from 10-day-old wild-type or TR
0/0 mice were injected
into irradiated adult mice. Eleven days after the grafting the adult recipient
mice (wild type or TR
0/0) reconstituted with wild-type or
TR
0/0 cells, as indicated, were sacrificed and the
hematopoietic reconstitution in the spleen was analyzed by examination of
spleen paraffin sections after hematoxilin and eosin coloration to show the
size of the hematopoietic colonies (colonies are delineated by white dotted
lines in A-C), and by examination of cytospin cells after neutral benzidine
and MGG staining. Arrows in D and F point to hemoglobin-positive
erythroblasts.