Fig. 1. PCR transgene analysis. (A) Semen from six chimeric G0 roosters was analyzed by PCR for the presence of the transgene. Three roosters were positive for the transgene in the germline, but only rooster 6 was able to pass the transgene onto his offspring. (B) Two mature offspring from rooster 6 had their semen tested for the presence of the transgene. Increasing template quantity from one of these G1 heterozygous roosters showed the expected quantity-dependent band increase. Neg, negative control; Pos, positive control; MW, molecular weight marker.