Fig. 3. E14 chick organs express GFP uniformly. Fresh organs from E14 embryos were dissected out and checked for GFP using fluorescence microscopy. Kidneys (A-C), eye (D-F), heart (G-I) and brain (J-L) are all positive for GFP expression. Lung, liver, pancreas, intestine and gizzard also uniformly express GFP (not shown). (C) Transverse section of the left kidney shown in A. (F) Magnified image of the right anterior portion of the transverse section of the eye in D. The heart in G was sectioned through the plane of the paper. L is an oblique section through the caudal part of the brain in J, including the tectum, cerebellum and brain stem. Lines in A,D and J indicate level of sections in C,F and L, respectively. a, apex; bs, brain stem; c, choroid; cb, cerebellum; ch, cerebral hemisphere; l, lens; ms, mesonephros; mt, metanephros; ot, outflow tract; r, retina; rpe, retinal pigment epithelium; s, sclera; t, tectum; v, ventricle. Scale bars: in A, 150 µm for A,B,D,E,G-K; in C, 50 µm; in F, 40 µm; in L, 35 µm.