Fig. 5. Somite graft using transgenic chick tissue. (A) Three somites from a transgenic embryo (black arrowheads) were grafted into the left side of a 48-hour wild-type chick host embryo (ventral side up in EC culture). (B) After a 24-hour incubation, the integrated graft was observed on the left side of the whole-mount embryo (dorsal view) after processing for immunocytochemistry with an anti-GFP antibody and DAB staining. (C) Higher magnification view of the integrated graft in B. (D) Section at the level indicated by the horizontal line in B. Paraffin section (12 µm) at the level of the donor somitic tissue demonstrates that cells integrate into the host embryo in the neural tube, dermomyotome and heart. Scale bars: in B, 200 µm for A,B; in C, 200 µm for C,D.