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Fig. 6. In situ hybridization (ISH) and GFP expression in wholemount embryos. Chd (A,B), Ganf (C,D) and Sox2 (E,F) were used as ISH probes in whole-mount embryos. Anti-GFP antibody with an Alexa Fluor® 488-tagged secondary antibody was used to restore fluorescence following ISH (B,D,F). Scale bar: in F, 200 µm for A-F.