Fig. 1. Activity of Wnt1-Cre or Isl1-Cre in peripheral nerves. The spinal cord and dorsal root ganglia (DRG) of E14.5 Rosa26 Cre-dependent lacZ reporter (R26R) line with Wnt1-Cre (A,C,E,G,I) or Isl1-Cre transgene (B,D,F,H,J) are shown. Triple immunofluorescence confocal microscopy with antibodies to ß-galactosidase (ß-gal) (red), the glial marker BFABP (blue) and the neuronal marker HuD (green) is shown. Open arrowheads indicate DRG and open arrows indicate motoneurons in the spinal cord. White arrows indicate Cre activation in motoneurons. Insets are higher-magnification details of the DRG. Scale bar: 100 µm. (K) Schematic illustrating peripheral nerve-specific Cre activation (blue).