Fig. 3. VEGFA is required for normal DRG vascularization. (A-F) Double immunofluoresence confocal microscopy of E15.5 trunk sections performed using antibodies to PECAM1 (A-D, blue) and HuD (A,B,E,F, green). (G) Quantification of length of PECAM1⁺ vascular branching in DRG. The internal vascularization of the DRG (C, arrow) is disrupted in the nerve-Vegfa conditional mutants (D), while vessels peripheral to the DRG appeared unaffected (C,D, arrowheads). Asterisk indicates a statistically significant difference (P<0.05) in Vegf^flox/flox compared with Wnt1-Cre; Isl1-Cre; Vegf^flox/flox (Student's t-test). Scale bar: 100 µm.