Fig. 4. Nerve-blood vessel alignment is unaffected in the absence of nerve-derived VEGFA. (A-H) Whole-mount triple immunofluorescence confocal microscopy using antibodies to BFABP (A-H, red), PECAM1 (A-H, blue) and neurofilament (2H3) (A-D, green) is shown. Close-up images (C,D,G,H) show the boxed regions in A,B,E,F. Arrows indicate blood vessels, arrowheads the aligned nerves and open arrowheads Schwann cells. Scale bars: 100 µm. (I) The alignment of nerves with blood vessels was quantified as the percentage of nerve length aligned with vessels.