Fig. 5. Reduction of VEGFA expression by Cre-mediated recombination. (A-H) Triple immunofluorescence confocal microscopy using antibodies to VEGF (A,B,E,F, red), HuD (C-F, green) and BFABP (G,H, blue). Arrows indicate DRG; arrowheads indicate spinal cord. (I) Quantification of anti-VEGFA immunoreactivity in DRG in embryos of various genotypes. There was no consistent alteration in either HuD⁺ neuronal (C,D) or BFABP⁺ glial (G,H) development in multiple specimens. Scale bars: 100 µm. (J) The efficient deletion of the floxed Vegf allele was determined by PCR analysis of DRG. The PCR product of the recombined allele is smaller than that of the non-recombined allele.