(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Intron 2 sequences are required for maintaining E-cadherin expression. (A-D) Whole-mount ß-gal staining of F1 embryos of CK14-Cre males crossed to Ecad-In2flox females. A slightly reduced staining is seen at E12.5 (A) in embryos where Cre was active (+/{Delta}) compared with control embryos with no Cre allele (+/flox). Further reduction is found in E13.5 (B), E14.5 (C) and E16.5 (D) embryos. Tissues where Cre was not active (lens, gut loops) are still strongly stained. (E,F) Whole-mount ß-gal staining of F1 embryos of CK19-Cre males crossed to Ecad-In2flox females. No difference in gene activity is observed at E9.5 (E), but decreased gene activity after intron 2 deletion is visible in E10.5 embryos (F). (G-J) Transverse sections of the gut tube (G,H) and sagittal sections of the pharynx (I,J) of whole-mount stained E10.5 control (+/flox, G,I) and Ecad-In2flox/CK19-Cre embryos (+/{Delta}, H,J). Scale bars: 500 µm in E; 1 mm in A,B,F; 2 mm in C,D; 50 µm in G-J.