Fig. 1. aPKC, Crumbs3 and Lgl2 show specific localisation in early epithelial cells. (A) GFP-Lgl2 localised exclusively to the basolateral membrane at stage 8. (B) Crumbs3-GFP localised to the apical (arrowhead) and basolateral membrane at stage 8, and to unknown internal structures (arrow). (C) GFP control. The examples shown are after injecting 1 ng of RNA. (D) RLDX control. GFP localised nonspecifically in the cytoplasm, nucleus and points of cell contact, as did the lineage label RLDX. Because of the high yolk content of early Xenopus cells, cytoplasmic fluorescence of the controls has a latticed appearance. This is very distinct from the localisation of the fusion proteins shown. (E) Antibody staining showing that aPKC localises to the apical membrane.