Fig. 7. aPKC and Lgl act by a process of mutual inhibition. (A,B) GFP-Lgl was injected on its own (A) or with aPKC (B). Addition of aPKC inhibited the basolateral localisation of GFP-Lgl2. GFP was visualised by using an anti-GFP antibody. (C,D) Overexpression of Lgl2 inhibited the apical localisation of aPKC but overexpression of GFP did not. (E-G) Lgl2, but not GFP injections, can rescue the apicalisation caused by injecting aPKC. There are more rounded cells in aPKC plus GFP-injected embryos than in aPKC plus Lgl2-injected embryos. The graph shows the average percent of embryos with apicalised cells from three experiments. The experiment was scored blind as for Fig. 2.