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Fig. 7. FGFR1 and FGFR2 are expressed throughout the epithelium. (A) FGFR1 staining (red) is increased around the edge of the bud with FGF7 treatment, and near the tip of the duct with FGF10 treatment. E-cadherin (green) defines the cell junctions of the epithelium, and SYBR-green stains the nucleus (pseudocolored blue). (B) FGFR2 staining (red) is localized throughout the epithelium in a punctate pattern and shows less cell membrane localization with FGF7 than with FGF10 treatment. E-cadherin (green) defines the cell junctions of the epithelium and SYBR-green stains the nucleus (pseudocolored blue). Scale bars: 20 µm.