Fig. 8. FGF7- and FGF10-mediated morphogenesis is ERK1/2 dependent. FGF7-mediated morphogenesis is also PI3K dependent. FGF7- (200 ng/ml) and FGF10- (1500 ng/ml) mediated morphogenesis is inhibited by the FGFR inhibitor SU5402 (SU, 2.5 µM), the MEK1/2 inhibitor UO126 (UO, 10 µM), and by rFGFR2b (R2b, 5 µg/ml), but not by the broad PKC inhibitor GO6983 (GO, 1.5 µM), or by rFGFR1b (R1b, 10 µg/ml). FGF7-mediated morphogenesis is also inhibited by the PI3K inhibitor LY294002 (LY, 10 µM). Star indicates an inhibited phenotype. The inhibitors were added to the media for 44 hours, and the number of buds (for FGF7) and the length of ducts (for FGF10) were measured using MetaMorph software and expressed as a ratio at 44 hours/time 0. ^*, P<0.5; ^**, P<0.01. See Movie 2 in supplementary material, which shows FGF7- and FGF10-treated epithelium cultured for 36 hours.