Fig. 5. td1 transcripts are decreased in a td1-Mu allele. (A) RNA gel blot of 2 µg polyA⁽⁺⁾ RNA from immature ears of td1-glf and normal siblings was hybridized to a td1 specific probe and revealed an abnormal sized transcript (asterisk). The same membrane was stripped and re-probed with the kn1 cDNA as a loading control. (B) Real-time RT-PCR also showed that the level of td1 RNA was reduced in td1-glf (20%) relative to wild type, whereas the level of the kn1 meristem marker was slightly increased.