Fig. 5. ntl and the Nodal signaling pathway regulate lrdr1 expression, and, with other genes, control morphogenesis of Kupffer's vesicle. Wild-type (A,F) and mutant ntl^b195(B,G), spt^b104(C,H), cas^ta56 (D,I) and oep^m134 (E,J) embryos were examined for lrdr1 mRNA expression, cilia formation and KV organization. (A-E) lrdr1 expression at 80-90% epiboly. Lateral views, dorsal towards the right, anterior upwards; arrows indicate DFCs. (F-J) Immunofluorescence analysis of cilia and KV morphogenesis using anti-acetylated Tubulin antibodies (cilia, green) and anti-Ntl antibodies (KV, red) at 6-10 SS. Dorsal views with anterior towards the left. Arrows indicate cilia.