Fig. 2. Electroporation of pCAG-ExZ, carrying EYFP and nlacZ, at two developmental stages. Coronal sections of the primary somatosensory area of P5 brains after electroporation at E13.5 (A-D,I-N), and E15.5 (E-H,O-Q) are shown. Green and blue signals show EYFP fluorescence (A,E), and immunostaining with an anti-ß-gal antibody (B,D,F,H,I,K,L,N,O,Q), respectively. Red signals show immunostaining with antibodies recognizing ER81 (C,D,J,K) and Lhx2 (G,H,M,N,P,Q). ß-Gal⁺ neurons of layer V, and of layers II and III, were positive for ER81 and Lhx2, respectively (arrowheads). ER81⁺ neurons expressed ER81 relatively uniformly at high levels, whereas 79.7±2.3% of ß-gal⁺/Lhx2⁺ neurons expressed Lhx2 at high levels. The ratio closely resembled that of strongly Lhx2-positive cells in the total population of Lhx2⁺ cells of layers II and II (78.6±2.7%). A-D, E-H, I-K, L-N, and O-Q each show the same section; D,H,K,N and Q are merged views. WM, white matter; LV, lateral ventricle. Scale bars: 200 µm for A-H; 20 µm for I-Q.