Fig. 1. Immunostaining of Wt1, neural cell adhesion molecule (NCAM) and glial fibrillary acidic protein (GFAP) in the olfactory epithelium of a wild-type mouse embryo at E18.5. Wt1 was detected in the nuclei of a significant proportion of cells at the base of the olfactory epithelium (a). Double-immunostaining of NCAM (green fluorescence) and Wt1 (red fluorescence) revealed an overlapping pattern of both proteins (Ac). By contrast, Wt1 and glial fibrillary acidic protein (GFAP) were not co-localized in cells of the olfactory epithelium (B). The results shown are representative for the more than 20 tissue sections from five different embryos. Scale bars: 50 µm.