Fig. 7. Representative HE-staining of the developing eyes of mouse embryos at E12.5 (A) and E18.5 (B). Strikingly, the Wt1(-KTS)-deficient embryos exhibited thinner retinas with fewer cells (A, parts c, d) than age-matched normal mice (A, parts a, b). For comparison, the ocular phenotype was less severe in embryos with inactivated Wt1(+KTS) (A, parts e, f). (B) Abnormal morphology of the retina, in particular the reduced cell density in the future ganglion cell layer, was clearly evident in Wt1(-KTS)-deficient embryos at E18.5 (part d; compare with b and f). Scale bars: 10 µm in B, parts b, d, f; 50 µm in A, parts b, d, f; 50 µm in B, parts a, c, e; 100 µm in A, parts a, c, e.