Fig. 3. Cell proliferation and apoptosis in Sftpc-NmycEGFP transgenic lungs. Sections of wild-type (A,C,E) and transgenic (B,D,F) E18.5 lung after a 1-hour pulse in vivo with BrdU followed by immunohistochemistry (A,B), or after staining with antibody to PHH3 (C,D) or cleaved caspase 3 (E,F). Note much higher rate of cell proliferation in both mesenchyme and epithelium of the transgenic lung, and the epithelial cells undergoing apoptosis (arrowheads). Scale bars: 50 µm.