Fig. 7. cos2 mutant clones and S182N-expressing clones at the AP boundary do not express anterior en. (A-D) cos2^- cells in a clone at the AP boundary lacks anterior en expression. A disc stained with anti-Myc (red) to reveal cos2^- clones, which are not Myc-positive and are therefore lack red signal (arrow), is co-stained with anti-En 4D9 (green). The area marked in A (white box) is shown at higher magnification in B-D. No En is detected within the clone (arrow). (E-G) Cells at the AP boundary expressing S182N-GFP (green) fail to express anterior En (blue, arrow). The area shown in E (white box) is shown at higher magnification in E and F.