Fig. 5. MIG-13 requires ceh-20 and unc-62, but not lin-39, to promote anterior migration. The left column shows the distribution of QR.pax cells in mig-13(mu225), lin-39(n1760), ceh-20(mu290) and unc-62(mu232) single mutants. Expression of mig-13 in all neurons under the unc-119 promoter (Punc-119::mig-13) rescues this migration defect in mig-13(-) and lin-39(-), but not in ceh-20(-) or unc-62(-) animals. In an otherwise wild-type background, Punc-119::mig-13 does not alter the distribution of these cells. The vertical gray line indicates the birthplace of the QR cell. The pink arrow indicates the total anterior distance traversed by QR and its descendants giving rise to QR.pax in wild-type animals. n=100 cells for each strain. The right column shows the distribution of the right BDU cell (BDUR) in these same strains at the end of L1. The embryonic migration of BDU starts from the region between V1 and V2 (indicated by the vertical gray line) and ends just anterior to V1 in wild-type animals. The full anterior migration of this cell is blocked in 8% of wild type, 92% of mig-13(mu225), 26% of lin-39(n1760), 82% of ceh-20(mu290) and 96% of unc-62(mu232) animals. Expression of mig-13 in all neurons restores full anterior migration in 88% and 86% of mig-13(mu225) and lin-39(n1760) animals, respectively, but in only 46% and 38% of ceh-20(mu290) and unc-62(mu232) animals, respectively. In an otherwise wild-type background, Punc-119::mig-13 does not alter the distribution of BDUR. Similar distributions were observed for the left BDU cell in these strains (data not shown). The black arrow indicates the anterior distance that BDU migrates in wild-type animals. n=50 cells for each strain.