Fig. 3. bap enhancer regions and reporter constructs from D. melanogaster (A) and D. virilis (B). A restriction map of the genomic locus including the bagpipe (bap) transcription unit (5' to the left) is shown at the top of each panel. Shown below are the genomic fragments tested in reporter constructs, with the arrowhead indicating their orientations in the construct (arrowheads point towards basal promoter). bapH2-1.2 (D. melanogaster), bapDS2.7-R (D. virilis) and their respective subfragments are shown at higher magnification. The in vivo reporter gene expression patterns are indicated on the right-hand side. Identified enhancer regions are summarized at the bottom of each panel. B, BamHI; R, EcoRI; H, HincII; S, SalI; ec, ectoderm; ms, mesoderm; FVM, foregut visceral mesoderm; HVM, hindgut visceral mesoderm; TVM, trunk visceral mesoderm.