Fig. 4. DNAseI protection experiments with candidate transacting factors on bap3.2.1 enhancer DNA. ³²P-labelled probe was tested with two different amounts (1x and 3x, see Materials and methods) of bacterially expressed GST-fusion proteins of Bap, Tin, Slp, Mad, Medea and Bin, as well as BSA as a control. C+T sequencing ladder is shown on the left of each blot and a schematic drawing of protected regions on the right.