Fig. 5. Differentiated lineage analysis in intestinal mucosa: commitment to the Paneth cell lineage. (A) Villin, UEA-1 and chromogranin staining in sections from control and mutant mice on day 4. Arrows indicate chromogranin-positive cells. (B) H&E and ß-catenin staining of the crypt compartment of control mice (a,f). Arrows indicate Paneth cells (a) and nuclear ß-catenin staining in the Paneth cells (f). In situ hybridization of lysozyme and cryptdins in the small intestine of control mice (b,g), in colon polyps from APC^+/- mice at low (c,h) and high magnification (d,i), and in the small intestine of mutant mice (e,j) at day 4.