Fig. 6. Cryptdin/defensin family genes are direct targets of ß-catenin signaling. (A) Consensus Tcf-binding sites (black boxes) are present in 300 bp regions of promoter sequences of distinct cryptdin and defensin genes. (B, left) Schematic representation of cryptdin/defensin promoter constructs showing the putative Tcf-binding sites (black boxes). (B, right) Responses of various promoter fragments (cryptdin5-2500, HD5-1000 and HD6-2500) to activated ß-catenin and Tcf4 in 293T cells (^*P<0.05). (C) HD6 promoter constructs of various lengths showing the putative Tcf-binding sites (left), mutated or not, and their response to ß-catenin/Tcf4 (right, ^*P<0.05).